Anti-BRD8/p120抗体
参阅全部 BRD8/p120 一抗
兔多克隆抗体to BRD8/p120
Rabbit
Specific to p120
适用于: IP, IHC-P, WBmore details
与反应: Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant
Synthetic peptide within BRD8/p120 aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9H0E9
Nuclear extract from HeLa cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Antibody was affinity purified using an epitope specific to BRD8/p120 immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab17969于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa). |
Entrez Gene: 10902 Human
Omim: 602848 Human
SwissProt: Q9H0E9 Human
Unigene: 519337 Human
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Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)
ab17969 immunoprecipitating BRD8 at 6 µg per reaction.
Lane 1: ab17969 in HeLa nuclear extract (0.5 or 1 mg per IP reaction; 20% of IP loaded).
Lane 2: IgG control
For blotting immunoprecipitated BRD8, ab17969 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 / p120 with ab17969 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-BRD8/p120 antibody (ab17969)
Detection of human BRD8 by Western Blot. Sample is nuclear
extract (NE; 5, 15, and 50mcg) from HeLa cells.
