Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade

• 产品名称

  Anti-Histone H4抗体[mAbcam 17036] - ChIP Grade
  参阅全部 Histone H4 一抗

• 描述

  小鼠单克隆抗体[mAbcam 17036] to Histone H4 - ChIP Grade

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, ChIP, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
  (Peptide available as ab13843)

• 阳性对照

• This antibody gave a positive signal in the following: Calf thymus histone preparation; HeLa histone preparation; Histone H4 recombinant protein; NIH3T3 Whole Cell Lysate; MEF1 Whole Cell Lysate; PC12 Whole Cell Lysate.

• 常规说明

  To detect histone H4 by ICC/IF, we recommend using ab177840 or ab9052 since these antibodies perform better in this application.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 纯度

  IgG fraction

• 克隆

  单克隆

• 克隆编号

  mAbcam 17036

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab17036于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Entrez Gene: 8364 Human

• Entrez Gene: 8365 Human

• Entrez Gene: 8366 Human

• Entrez Gene: 8367 Human

• Entrez Gene: 8368 Human

• Entrez Gene: 8370 Human

• Entrez Gene: 100041230 Mouse

• Entrez Gene: 319155 Mouse

• Entrez Gene: 319156 Mouse

• Entrez Gene: 319157 Mouse

• Entrez Gene: 319158 Mouse

• Entrez Gene: 319159 Mouse

• Entrez Gene: 319160 Mouse

• Entrez Gene: 319161 Mouse

• Entrez Gene: 320332 Mouse

• Entrez Gene: 326619 Mouse

• Entrez Gene: 326620 Mouse

• Entrez Gene: 674678 Mouse

• Entrez Gene: 69386 Mouse

• Entrez Gene: 97122 Mouse

• Entrez Gene: 64627 Rat

• Omim: 142750 Human

• Omim: 602822 Human

• Omim: 602823 Human

• Omim: 602824 Human

• Omim: 602825 Human

• Omim: 602826 Human

• Omim: 602827 Human

• Omim: 602828 Human

• Omim: 602829 Human

• Omim: 602830 Human

• Omim: 602831 Human

• Omim: 602833 Human

• SwissProt: P62805 Human

• SwissProt: P62806 Mouse

• SwissProt: P62804 Rat

• Unigene: 143080 Human

• Unigene: 247816 Human

• Unigene: 248172 Human

• Unigene: 248178 Human

• Unigene: 248179 Human

• Unigene: 278483 Human

• Unigene: 46423 Human

• Unigene: 528055 Human

• Unigene: 533295 Human

• Unigene: 55468 Human

• Unigene: 591790 Human

• Unigene: 655235 Human

• Unigene: 662174 Human

• Unigene: 706635 Human

• Unigene: 718935 Human

• Unigene: 144300 Mouse

• Unigene: 14775 Mouse

• Unigene: 158272 Mouse

• Unigene: 227295 Mouse

• Unigene: 228709 Mouse

• Unigene: 246720 Mouse

• Unigene: 255646 Mouse

• Unigene: 260530 Mouse

• Unigene: 261642 Mouse

• Unigene: 261662 Mouse

• Unigene: 261664 Mouse

• Unigene: 377875 Mouse

• Unigene: 442307 Mouse

• Entrez Gene: 121504 Human

• Entrez Gene: 554313 Human

• Entrez Gene: 8294 Human

• Entrez Gene: 8359 Human

• Entrez Gene: 8360 Human

• Entrez Gene: 8361 Human

• Entrez Gene: 8362 Human

• Entrez Gene: 8363 Human

• 别名

• dJ221C16.9 antibody

• FO108 antibody

• H4 antibody

• H4.k antibody

• H4/a antibody

• H4/b antibody

• H4/c antibody

• H4/d antibody

• H4/e antibody

• H4/g antibody

• H4/h antibody

• H4/I antibody

• H4/j antibody

• H4/k antibody

• H4/m antibody

• H4/n antibody

• H4/p antibody

• H4_HUMAN antibody

• H4F2 antibody

• H4F2iii antibody

• H4F2iv antibody

• H4FA antibody

• H4FB antibody

• H4FC antibody

• H4FD antibody

• H4FE antibody

• H4FG antibody

• H4FH antibody

• H4FI antibody

• H4FJ antibody

• H4FK antibody

• H4FM antibody

• H4FN antibody

• H4M antibody

• HIST1H4A antibody

• HIST1H4B antibody

• HIST1H4C antibody

• HIST1H4D antibody

• HIST1H4E antibody

• HIST1H4F antibody

• HIST1H4H antibody

• HIST1H4I antibody

• HIST1H4J antibody

• HIST1H4K antibody

• HIST1H4L antibody

• HIST2H4 antibody

• HIST2H4A antibody

• Hist4h4 antibody

• Histone 1 H4a antibody

• Histone 1 H4b antibody

• Histone 1 H4c antibody

• Histone 1 H4d antibody

• Histone 1 H4e antibody

• Histone 1 H4f antibody

• Histone 1 H4h antibody

• Histone 1 H4i antibody

• Histone 1 H4j antibody

• Histone 1 H4k antibody

• Histone 1 H4l antibody

• Histone 2 H4a antibody

• histone 4 H4 antibody

• Histone H4 antibody

• MGC24116 antibody

• dJ160A22.1 antibody

• dJ160A22.2 antibody

• dJ221C16.1 antibody

• 

  ChIP - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

  Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab17036 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

  IHC image of ab17036 staining in Human Hodgekin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17036, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

  All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 2 µg/ml
  
  Lane 1 : Histone H1 Recombinant Protein
  Lane 2 : Histone H2A Recombinant Protein
  Lane 3 : Histone H2B Recombinant Protein
  Lane 4 : Histone H3.1 Recombinant Protein
  Lane 5 : Histone H4 Recombinant Protein
  
  Lysates/proteins at 0.1 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 11 kDa
  Observed band size: 11 kDa
  
  
  Exposure time: 30 seconds
  

• 

  Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

  All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 1 µg/ml
  
  Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 11 kDa
  Observed band size: 14 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 1 minute
  

• 

  Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

  All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 5 µg/ml
  
  Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
  Lane 2 : HeLa Histone Preparation Nuclear Lysate at 2.5 µg
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 11 kDa
  Observed band size: 14 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 1 minute
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

  IHC image of Histone H4 staining in formalin fixed, paraffin embedded human Hodgkin's lymphoma tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17036, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036)

  All lanes : Anti-Histone H4 antibody [mAbcam 17036] - ChIP Grade (ab17036) at 5 µg/ml
  
  Lane 1 : Histone H1 Recombinant Protein
  Lane 2 : Histone H2A Recombinant Protein
  Lane 3 : Histone H2B Recombinant Protein
  Lane 4 : Histone H3.1 Recombinant Protein
  Lane 5 : Histone H4 Recombinant Protein
  
  Lysates/proteins at 0.1 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 11 kDa
  Observed band size: 14 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 30 seconds