Anti-Ctip2 antibody [25B6]

• 产品名称

  Anti-Ctip2抗体[25B6]
  参阅全部 Ctip2 一抗

• 描述

  大鼠单克隆抗体[25B6] to Ctip2

• 宿主

  Rat

• 特异性

  Detects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.

• 经测试应用

  适用于: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  不适用于: IHC-Fr

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant fragment corresponding to Human Ctip2. GTS fusion
  Database link: Q9C0K0

• 表位

  Between amino acids 1-150 of CTIP2.

• 阳性对照

• Flow Cyt: Jurkat cells. ICC/IF: Neonatal mouse hippocampal cultured neurons, Jurkat cells. WB: Nuclear extract from Jurkat cells; Mouse brain tissue lysate.

• 常规说明

  Hybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.50
  Preservative: 0.02% Sodium azide
  Constituents: 0.357% HEPES, 0.87% Sodium chloride

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Multi-step Chromatography

• 克隆

  单克隆

• 克隆编号

  25B6

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab18465于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IHC-Fr.

• 数据库链接

• Entrez Gene: 64919 Human

• Entrez Gene: 58208 Mouse

• Omim: 606558 Human

• SwissProt: Q9C0K0 Human

• SwissProt: Q99PV8 Mouse

• Unigene: 709690 Human

• Unigene: 392694 Mouse

• 别名

• ATL1 gamma antibody

• ATL1-delta antibody

• B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody

• B cell lymphoma/leukemia 11B antibody

• B-cell CLL/lymphoma 11B (zinc finger protein) antibody

• B-cell CLL/lymphoma 11B antibody

• B-cell lymphoma/leukemia 11B antibody

• BC11B_HUMAN antibody

• BCL-11B antibody

• Bcl11b antibody

• BCL11B/TRDC fusion antibody

• COUP TF interacting protein 2 antibody

• COUP-TF interacting protein 2 antibody

• COUP-TF-interacting protein 2 antibody

• Ctip 2 antibody

• CTIP-2 antibody

• CTIP2 antibody

• hRIT1 alpha antibody

• hRit1 antibody

• Radiation induced tumor suppressor gene 1 antibody

• Radiation induced tumor suppressor gene 1 protein antibody

• Radiation-induced tumor suppressor gene 1 protein antibody

• Rit 1 antibody

• Rit1 antibody

• zinc finger protein hRit1 alpha antibody

• ZNF856B antibody

• ATL1 alpha antibody

• ATL1 antibody

• ATL1 beta antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] (ab18465)

  Immunofluorescence staining of Ctip2 using ab18465 in Jurkat cells (+ve expression control, top panel) and Daudi cells (-ve expression control, bottom panel). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton-X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab18465 at 0.2 µg/mL and ab6046 at 1 µg/mL overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150084) (shown in red), both at 1/1000. Nuclear DNA was labelled with DAPI (shown in blue).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (ab18465)

  IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse hippocampus performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] (ab18465)

  Immunofluorescence staining of Ctip2 using ab18465 in Jurkat cells (+ve expression control, top panel) and Daudi cells (-ve expression control, bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton-X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab18465 at 0.2 µg/mL and ab6046 at 1 µg/mL overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150084) (shown in red), both at 1/1000. Nuclear DNA was labelled with DAPI (shown in blue).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (ab18465)

  IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human hippocampus* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

  Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.

• 

  Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

  Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody.
  
  Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

  Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

  Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

  Red is beta tubulin staining.
  Green is ab18465.

• 

  Flow Cytometry - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

  Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Western blot - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)This image is courtesy of an Anonymous Abreview.

  All lanes : Anti-Ctip2 antibody [25B6] (ab18465) at 1/500 dilution
  
  Lanes 1-2 : Mouse brain tissue lysate at 1.5 µg
  Lane 3 : Mouse brain tissue lysate at 3 µg
  
  Secondary
  All lanes : IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 95 kDa
  Observed band size: 100,110 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 10 minutes
  

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (ab18465)

  Negative control image: IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse cerebellum performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (ab18465)

  Negative control image: IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human cerebellum* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre