Anti-Leptin抗体
参阅全部 Leptin 一抗
兔多克隆抗体to Leptin
Rabbit
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Mouse, Human, Recombinant fragment
预测可用于: Goat, Horse, Cow, Cat, Dog, Chimpanzee, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human Leptin aa 91-106.
Sequence:
SRNVIQISNDLENLRD
Database link: P41159
Human Serum
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituent: PBS
Ammonium Sulphate Precipitation
多克隆
IgG
Abpromise™承诺保证使用ab16227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/20 - 1/200. | |
| WB | (1) | 1/500 - 1/5000. Detects a band of approximately 16 kDa. |
| IHC-P | (1) | Use at an assay dependent concentration. |
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SwissProt: Q257X2 Goat
SwissProt: Q95189 Gorilla
SwissProt: Q9TU09 Horse
SwissProt: P41159 Human
SwissProt: P41160 Mouse
Unigene: 194236 Human
Unigene: 277072 Mouse
Entrez Gene: 449638 Chimpanzee
Entrez Gene: 3952 Human
Entrez Gene: 16846 Mouse
Omim: 164160 Human
SwissProt: O02750 Chimpanzee
LEPD antibody
Leptin (murine obesity homolog) antibody
Leptin (obesity homolog, mouse) antibody
Leptin antibody
Leptin Murine Obesity Homolog antibody
Leptin Precursor Obesity Factor antibody
OB antibody
Obese protein antibody
Obese, mouse, homolog of antibody
Obesity antibody
Obesity factor antibody
Obesity homolog mouse antibody
Obesity Murine Homolog Leptin antibody
OBS antibody
OTTHUMP00000212285 antibody
FLJ94114 antibody
LEP antibody
LEP_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab16227)
Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin antibody (ab16227)
Ab16227 staining human placenta.
Left panel: with primary antibody. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Anti-Leptin antibody (ab16227)
Anti-Leptin antibody (ab16227) at 1/500 dilution + Recombinant Human Leptin protein (ab51273) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes
Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab16227)
Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab16227)
Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
