Anti-p53 antibody [SP5]

• 产品名称

  Anti-p53抗体[SP5]
  参阅全部 p53 一抗

• 描述

  兔单克隆抗体[SP5] to p53

• 宿主

  Rabbit

• 经测试应用

  适用于: IHC-P, Flow Cyt (Intra), ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein within Human p53. The exact sequence is proprietary.
  Database link: P04637

• 表位

  Not determined

• 阳性对照

• IHC-P: Colon carcinoma tissues Flow Cyt: HAP1 and MCF7 cells. ICC/IF: MCF7 cells. ICC/IF KO: Hap1 cells (Hap1-TP53 KO used as a negative cell line)

• 常规说明

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.1% Sodium azide
  Constituents: 1% BSA, 98% PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  SP5

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16665于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7157 Human

• Omim: 191170 Human

• SwissProt: P04637 Human

• Unigene: 654481 Human

• 别名

• FLJ92943 antibody

• LFS1 antibody

• Mutant tumor protein 53 antibody

• p53 antibody

• p53 tumor suppressor antibody

• P53_HUMAN antibody

• Phosphoprotein p53 antibody

• Tp53 antibody

• Transformation related protein 53 antibody

• TRP53 antibody

• tumor antigen p55 antibody

• Tumor protein 53 antibody

• Tumor protein p53 antibody

• Tumor suppressor p53 antibody

• Antigen NY-CO-13 antibody

• BCC7 antibody

• Cellular tumor antigen p53 antibody

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] (ab16665)

  Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

• Flow Cytometry (Intracellular) - Anti-p53 antibody [SP5] (ab16665)

  This image was generated from the hybridoma version of the product

  Flow cytometry overlay histogram showing wild-type HAP1 (green line) and TP53 knockout HAP1 cells stained with ab16665 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1%PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab16665) (1x106 in 100 µl at 0.008 µg/ml) for 30 min at 22°C.

  The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 22°C.

  Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line TP53 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min) / permeabilized with 0.1%PBS-Triton X-100 for 15 min used under the same conditions.

   

• Flow Cytometry (Intracellular) - Anti-p53 antibody [SP5] (ab16665)

  This image was generated from the hybridoma version of the product

  Flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling p53 with purified ab16665 at 1/200 dilution (0.81 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730)(black). Unlableled control - Unlabelled cells (blue).

• Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [SP5] (ab16665)

  ab16665 staining p53 in wild-type Hap1 cells (top panel) and p53 knockout Hap1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab16665 at 1/25 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo color red).  Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a high-content analysis system (Perkin Elmer, Operetta CLS™).

  This image was generated from the hybridoma version of the product

• Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [SP5] (ab16665)

  Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling p53 with purified ab16665 at 1/25 (6.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  This image was generated from the hybridoma version of the product