Anti-CD23抗体[SP23]
参阅全部 CD23 一抗
兔单克隆抗体[SP23] to CD23
Rabbit
适用于: WB, IHC-P, Flow Cytmore details
与反应: Human
Recombinant fragment within Human CD23 aa 1-250. The exact sequence is proprietary.
Database link: P06734
IHC-P: Human tonsil. Flow Cyt: Jurkat cell lysate. WB: Raji cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Protein A purified
单克隆
SP23
IgG
Abpromise™承诺保证使用ab16702于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use at an assay dependent concentration. | |
IHC-P | 1/100. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. | |
Flow Cyt | Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 2208 Human
Omim: 151445 Human
SwissProt: P06734 Human
Unigene: 465778 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (ab16702)
Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD23 with ab16702 at 1/100 (76 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16702 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.
Flow Cytometry - Anti-CD23 antibody [SP23] (ab16702)
This image was generated using a previous batch manufactured using hybridoma production method.
Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).
Western blot - Anti-CD23 antibody [SP23] (ab16702)
Anti-CD23 antibody [SP23] (ab16702) at 1/25 dilution + lysate prepared from Raji cells
Observed band size: 45 kDawhy is the actual band size different from the predicted?
This image was generated using a previous batch manufactured using hybridoma production method.