Anti-Synaptophysin antibody [SP11]

• 产品名称

  Anti-Synaptophysin抗体[SP11]
  参阅全部 Synaptophysin 一抗

• 描述

  兔单克隆抗体[SP11] to Synaptophysin

• 宿主

  Rabbit

• 经测试应用

  适用于: ICC, WB, IHC-P, IHC-Frmore details

• 种属反应性

  与反应: Rat, Human
  

• 免疫原

  Synthetic peptide within Human Synaptophysin aa 250 to the C-terminus. The exact sequence is proprietary.
  Database link: P08247

• 表位

  C-terminus

• 阳性对照

• WB: Human Brain lysate. IHC-Fr: Human Pancreas tissue sections. ICC: Primary hippocampal rat neurons/glia cells. IHC: human neuroendocrine tumor and Human pancreas.

• 常规说明

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.50
  Preservative: 0.1% Sodium azide
  Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  SP11

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16659于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6855 Human

• Entrez Gene: 24804 Rat

• Omim: 313475 Human

• SwissProt: P08247 Human

• SwissProt: P07825 Rat

• Unigene: 632804 Human

• Unigene: 11067 Rat

• 别名

• Syn p38 antibody

• Synaptophysin antibody

• Syp antibody

• SYPH antibody

• SYPH_HUMAN antibody

• SypI antibody

• Major synaptic vesicle protein p38 antibody

• MRX96 antibody

• MRXSYP antibody

• 

  Immunocytochemistry - Anti-Synaptophysin antibody [SP11] (ab16659)

  ab16659 staining Synaptophysin in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16659 at a 1/100 dilution and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Also suitable in cells fixed with 4% paraformaldehyde (10 min).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SP11] (ab16659)

  Immunohistochemical staining of human neuroendocrine tumor with ab16659.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SP11] (ab16659)

  Immunohistochemical staining of human pancreas with ab16659.

• 

  Immunohistochemistry (Frozen sections) - Anti-Synaptophysin antibody [SP11] (ab16659)

  IHC image of Synaptophysin staining in a section of frozen normal human pancreas performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab16659, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-Synaptophysin antibody [SP11] (ab16659)

  Anti-Synaptophysin antibody [SP11] (ab16659) at 1/500 dilution + Lysate prepared from human brain
  
  Predicted band size: 38 kDa