Anti-Noggin抗体
参阅全部 Noggin 一抗
兔多克隆抗体to Noggin
Rabbit
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab124977.
适用于: IHC-P, WBmore details
不适用于: IHC-Fr
与反应: Mouse, Human
预测可用于: Horse, Chicken, Xenopus laevis
Synthetic peptide corresponding to Human Noggin aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab16380)
This antibody gave a positive signal in both Human and Mouse Noggin Recombinant protein.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16054于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (4) | 1/175. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
| WB | (2) | Use a concentration of 1 µg/ml. Detects a band of approximately 26, 35 kDa (predicted molecular weight: 26 kDa). |
应用说明
Is unsuitable for IHC-Fr.
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Unigene: 135266 Mouse
Unigene: 834 Xenopus laevis
Entrez Gene: 9241 Human
Entrez Gene: 18121 Mouse
Entrez Gene: 373646 Xenopus laevis
Omim: 602991 Human
SwissProt: Q13253 Human
SwissProt: P97466 Mouse
SwissProt: P49011 Xenopus laevis
Unigene: 248201 Human
SYM 1 antibody
SYM1 antibody
Symphalangism 1 (proximal) antibody
Synostoses (multiple) syndrome 1 antibody
SYNS 1 antibody
SYNS1 antibody
Nog antibody
NOGG_HUMAN antibody
Noggin antibody
Western blot - Anti-Noggin antibody (ab16054)
All lanes : Anti-Noggin antibody (ab16054) at 1 µg/ml
Lane 1 : Noggin Human Recombinant Protein
Lane 2 : Noggin Mouse Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26,35 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
Western blot - Anti-Noggin antibody (ab16054)
All lanes : Anti-Noggin antibody (ab16054) at 1 µg/ml
Lane 1 : Noggin Mouse Recombinant Protein
Lane 2 : Noggin Mouse Recombinant Protein with Human Noggin peptide (ab16380) at 1 µg/ml
Lysates/proteins at 0.01 µg per lane.
Predicted band size: 26 kDa
Western blot - Anti-Noggin antibody (ab16054)
Anti-Noggin antibody (ab16054) at 1 µg/ml + Recombinant human Noggin protein (ab73756) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Exposure time: 4 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noggin antibody (ab16054)
ab16054 stainning Noggin in paraffin-embedded human liver tissue, showing a cytoplasmic and/or membranous distribution in both hepatocytes and bile duct cells. Paraffin embedded tissue was incubated with ab16054 (1/175 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16054 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noggin antibody (ab16054)This image is courtesy of an anonymous Abreview.
Immunohistochemical analysis of human small intestine tissue, labeling Noggin with ab16054. Tissue was formaldehyde fixed, treated with EDTA (pH 8.6) at 100°C for 20 minutes for heat-mediated antigen retrieval and blocked with 3% Hydrogen Peroxide for 10 minutes at 25°C. Incubation with ab16054 (diluted 1/400) was performed for 20 minutes at 25°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noggin antibody (ab16054)This image is courtesy of an anonymous Abreview.
Immunohistochemical analysis of mouse kidney tissue, labeling Noggin with ab16054. Tissue was paraformaldehyde fixed, treated with Citrate buffer for heat-mediated antigen retrieval and blocked with Serum Free Protein Block for 20 minutes. Incubation with ab16054 (diluted 1/2500) was performed for 15 hours at 4°C
