Anti-MRP2 antibody [M2III-5]

• 产品名称

  Anti-MRP2抗体[M2III-5]
  参阅全部 MRP2 一抗

• 描述

  小鼠单克隆抗体[M2III-5] to MRP2

• 宿主

  Mouse

• 特异性

  This antibody does not cross-react with the human MDR1 P-gp, MRP1, MRP3, or MRP5 gene products.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  不与反应: Mouse

• 免疫原

  Recombinant fragment (MBP-tag) corresponding to Rat MRP2. This antibody was raised against a fusion protein of the bacterial maltose binding protein and rat MRP2, containing the 202-amino acid C terminal end of the transporter protein.

• 表位

  ab15603 reacts with an internal epitope of MRP2; a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.1% Sodium azide
  Constituents: Tissue culture supernatant, 0.7% BSA

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  M2III-5

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab15603于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1244 Human

• Omim: 601107 Human

• SwissProt: Q92887 Human

• Unigene: 368243 Human

• 别名

• ATP binding cassette subfamily C member 2 antibody

• ATP-binding cassette sub-family C member 2 antibody

• Canalicular multidrug resistance protein antibody

• Canalicular multispecific organic anion transporter 1 antibody

• CMOAT antibody

• CMOAT1 antibody

• cMRP antibody

• DJS antibody

• KIAA1010 antibody

• MRP 2 antibody

• MRP2_HUMAN antibody

• Multidrug resistance associated protein 2 antibody

• Multidrug resistance-associated protein 2 antibody

• ABC30 antibody

• abcC2 antibody

• ATP binding cassette sub family C (CFTR/MRP) member 2 antibody

• 

  Western blot - Anti-MRP2 antibody [M2III-5] (ab15603)

  All lanes : Anti-MRP2 antibody [M2III-5] (ab15603) at 1/20 dilution
  
  Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
  Lane 2 : ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 185.4 kDa
  
  
  
  

  Lanes 1 - 2: Merged signal (red and green). Green - ab15603 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.

  ab15603 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab15603 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.