Anti-Fragilis抗体
参阅全部 Fragilis 一抗
兔多克隆抗体to Fragilis
Rabbit
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: IP, ICC/IF, IHC-Fr, WBmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ICC/IF: mES cells. IHC-Fr: E12.5 mouse embryos. WB: Mouse embryonic stem cell, Mouse embryonic germ cell lysates. IP: E14Tg2a whole cell extract.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15592于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC/IF | (1) | Use a concentration of 1 - 10 µg/ml. |
| IHC-Fr | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). |
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Unigene: 141021 Mouse
Unigene: 145191 Rat
Entrez Gene: 10410 Human
Entrez Gene: 66141 Mouse
Omim: 605579 Human
SwissProt: Q01628 Human
SwissProt: Q9CQW9 Mouse
Unigene: 374650 Human
IFM3_HUMAN antibody
Interferon induced transmembrane protein 3 (1 8U) antibody
Interferon Induced Transmembrane Protein 3 antibody
Interferon inducible antibody
Interferon inducible protein 1 8U antibody
Interferon Inducible Protein 15 antibody
Interferon Inducible Protein Homolog antibody
Interferon-induced transmembrane protein 3 antibody
Interferon-inducible protein 1-8U antibody
IP15 antibody
1 8U antibody
Fragilis antibody
IFITM3 antibody
Immunocytochemistry/ Immunofluorescence - Anti-Fragilis antibody (ab15592)
ab15592 staining Fragilis in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab15592 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Western blot - Anti-Fragilis antibody (ab15592)
All lanes : Anti-Fragilis antibody (ab15592) at 1 µg/ml
Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Stem Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell lysate with Fragilis peptide (ab15737) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa
Immunohistochemistry (Frozen sections) - Anti-Fragilis antibody (ab15592)This image is courtesy of Petra Hajkova, University of Cambridge
IHC showing localisation of Fragilis (red) in cryosections of male genital ridges of E12.5 mouse embryos. Germ cells can be identified by staining with anti-Oct4 (green).
ab15592 (anti-Fragilis) = red
Oct4 = green
DAPI (DNA) = blue
The dilution of ab15592 used was 10 ug/ml.
Immunoprecipitation - Anti-Fragilis antibody (ab15592)
Fragilis - Primordial Germ Cell Marker was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5µg of Rabbit polyclonal to Fragilis and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15592.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 13kDa: Fragilis - Primordial Germ Cell Marker.
