Anti-Peroxiredoxin 1/PAG抗体
参阅全部 Peroxiredoxin 1/PAG 一抗
兔多克隆抗体to Peroxiredoxin 1/PAG
Rabbit
This antibody detects Peroxiredoxin 1 protein in human samples. The antibody is specific for Peroxiredoxin 1/PAG and shows no cross reactivity with other Prx isoforms.
适用于: ICC/IF, IHC-P, WBmore details
与反应: Human, African green monkey
预测可用于: Cow, Chinese hamster
Synthetic peptide corresponding to Human Peroxiredoxin 1/PAG aa 103-114.
Sequence:
LVSDPKRTIAQD
Recombinant human Peroxiredoxin 1/PAG protein (ab74172) can be used as a positive control in WB. TSU Pr1 cells for cell staining, human PC3 cell lysate for western blotting.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab15571于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | 1/200. |
| IHC-P | (1) | Use at an assay dependent concentration. |
| WB | (7) | 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa). |
Entrez Gene: 5052 Human
Omim: 176763 Human
SwissProt: Q9JKY1 Chinese hamster
SwissProt: Q06830 Human
Unigene: 180909 Human
NKEF A antibody
NKEF-A antibody
NKEFA antibody
OSF3 antibody
Osteoblast specific factor 3 antibody
PAG antibody
Paga antibody
PAGB antibody
Peroxiredoxin 1 antibody
Peroxiredoxin-1 antibody
PRDX1 antibody
PRDX1_HUMAN antibody
Proliferation associated gene A antibody
Proliferation-associated gene protein antibody
PRX1 antibody
PrxI antibody
TDPX2 antibody
Thioredoxin peroxidase 2 antibody
Thioredoxin-dependent peroxide reductase 2 antibody
Heme binding 23 kDa protein antibody
MSP23 antibody
Natural killer cell-enhancing factor A antibody
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab15571 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab15571 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab15571 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
Immunocytochemistry/ Immunofluorescence analysis of Peroxiredoxin 1/PAG was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab15571 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300. Panel d represents the merged image showing cytosolic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab15571) at 1/250 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : Ramos whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
ICC/IF image of ab15571 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15571, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
IHC image of ab15571 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15571, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab15571) at 1/1000 dilution
Lane 1 : Cell line indicated at 25 µg
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 20 kDawhy is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Western blot analysis of Peroxiredoxin 1//PAG was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab15571 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and chemiluminescent detection performed.
