Anti-beta Tubulin抗体
参阅全部 beta Tubulin 一抗
兔多克隆抗体to beta Tubulin
Rabbit
Homology with Tubulin beta chain and Tubulin beta-3 chain
适用于: ICC/IF, IHC-P, WBmore details
与反应: Human
预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis
Synthetic peptide within Human beta Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q13509
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
500 µl 浓度为 0.09 mg/ml
1 ml 浓度为 0.09 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15568于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | Use a concentration of 1 µg/ml. |
| IHC-P | (2) | 1/200. Antigen retrieval is not essential but may optimise staining. |
| WB | (3) | Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
Entrez Gene: 396254 Chicken
Entrez Gene: 203068 Human
Entrez Gene: 22154 Mouse
Entrez Gene: 380418 Xenopus laevis
Omim: 191130 Human
SwissProt: P07437 Human
SwissProt: P99024 Mouse
SwissProt: Q91575 Xenopus laevis
Unigene: 636480 Human
Unigene: 46552 Xenopus laevis
Beta 4 tubulin antibody
Beta 5 tubulin antibody
beta Ib tubulin antibody
Beta1 tubulin antibody
Class I beta tubulin antibody
M40 antibody
MGC117247 antibody
MGC16435 antibody
OK/SW cl.56 antibody
OK/SWcl.56 antibody
TBB5_HUMAN antibody
TUBB 1 antibody
TUBB 2 antibody
TUBB 5 antibody
TUBB antibody
TUBB1 antibody
TUBB2 antibody
TUBB5 antibody
tubulin beta 1 chain antibody
Tubulin beta 2 chain antibody
tubulin beta 5 chain antibody
Tubulin beta chain antibody
Tubulin beta class I antibody
tubulin beta polypeptide antibody
Tubulin beta-5 chain antibody
Western blot - Anti-beta Tubulin antibody (ab15568)
All lanes : Anti-beta Tubulin antibody (ab15568) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody (ab15568)
ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody (ab15568)This image is courtesy of an anonymous Abreview
ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody (ab15568)
ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).
Western blot - Anti-beta Tubulin antibody (ab15568)
