Anti-Bcl3抗体[Ham150-3.5]
参阅全部 Bcl3 一抗
仓鼠单克隆抗体[Ham150-3.5] to Bcl3
Hamster
ab15046 recognises Bcl3 from transfected cells and shows no cross reactivity to other Bcl isoforms.
适用于: WB, Flow Cytmore details
与反应: Mouse, Human
Full length protein corresponding to Mouse Bcl3.
C57BI/6 human T cells and Bcl3 transgenic ms T cells. Flow Cyt: BAF3, Ramos cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Protein G purified
单克隆
Ham150-3.5
IgG
Abpromise™承诺保证使用ab15046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 50 kDa). | |
Flow Cyt | Use at an assay dependent concentration. 1 µg/test (100µl) |
Entrez Gene: 602 Human
Entrez Gene: 12051 Mouse
Omim: 109560 Human
SwissProt: P20749 Human
SwissProt: Q9Z2F6 Mouse
Unigene: 31210 Human
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Flow Cytometry - Anti-Bcl3 antibody [Ham150-3.5] (ab15046)
Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
Flow Cytometry - Anti-Bcl3 antibody [Ham150-3.5] (ab15046)
Flow cytometric analysis of 2% paraformaldehyde-fixed BAF3cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
Western blot - Anti-Bcl3 antibody [Ham150-3.5] (ab15046)
Anti-Bcl3 antibody [Ham150-3.5] (ab15046) at 2 µg/ml
Predicted band size: 50 kDa
Western blot of Bcl3 in transgenic mouse T cells using Ab15046.