Anti-CRALBP antibody [B2]

• 产品名称

  Anti-CRALBP抗体[B2]
  参阅全部 CRALBP 一抗

• 描述

  小鼠单克隆抗体[B2] to CRALBP

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Rat
  预测可用于: Cow, Human, Pig, Monkey

• 免疫原

  Recombinant full length protein corresponding to Human CRALBP.
  Database link: P12271

• 阳性对照

• This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina. FFPE Mouse eye tissue sections.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  B2

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab15051于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: Q9Z275 Mouse

• Unigene: 1933 Human

• Unigene: 41653 Mouse

• Entrez Gene: 282038 Cow

• Entrez Gene: 6017 Human

• Entrez Gene: 19771 Mouse

• Entrez Gene: 100521278 Pig

• Entrez Gene: 293049 Rat

• Omim: 180090 Human

• SwissProt: P10123 Cow

• SwissProt: P12271 Human

• 别名

• MGC3663 antibody

• Retinaldehyde binding protein 1 antibody

• Retinaldehyde-binding protein 1 antibody

• RLBP 1 antibody

• RLBP1 antibody

• RLBP1_HUMAN antibody

• Cellular retinaldehyde binding protein 1 antibody

• Cellular retinaldehyde binding protein antibody

• Cellular retinaldehyde-binding protein antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [B2] (ab15051)

  Immunohistochemistry image of CRALBP staining in a section of formalin-fixed paraffin-embedded normal mouse eye performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15051, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-CRALBP antibody [B2] (ab15051)

  All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml
  
  Lane 1 : Rat eye tissue lysate - total protein (ab4035)
  Lane 2 : Rat retina tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 36 kDa
  Observed band size: 35 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 30 seconds