MCF7核extract裂解物(H2O2 post treated)
参阅全部 MCF7 细胞裂解液
Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.
Yes
Liquid
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
浓度
250 µg 浓度为 2.5 mg/ml
Functional Studies - MCF7 nuclear extract lysate (H2O2 post treated) (ab14863)
Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5 µg/well must be above 4.
