MCF7核extract裂解物(H2O2 treated)
参阅全部 MCF7 细胞裂解液
The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with or without 0.2 mM hydrogen peroxide. The protein content has been determined by a Bradford-based assay.
Yes
Liquid
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
浓度
100 µg 浓度为 2.5 mg/ml
Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (ab14862)
Each lot has been tested for p53 activation by using TransAM™ p53 Kit. The signal intensity for p53 activation in each lot is compared to the signal intensity obtained with extracts from untreated cells. Once the signals are blanked, the ratio between the signals from extracts of treated cells over untreated cells used at 5 µg/well is above 4. This ratio may vary depending on the basal level of p53 activation in a given cell type.
