Anti-RANK antibody [64C1385]

• 产品名称

  Anti-RANK抗体[64C1385]
  参阅全部 RANK 一抗

• 描述

  小鼠单克隆抗体[64C1385] to RANK

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, IPmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant fragment within Human RANK. The exact sequence is proprietary. Recombinant fragment containing the extracellular domain (amino acids 33-208) of human RANK.

• 阳性对照

• WB: RAW (a mouse macrophage monocyte). IHC-P: Human T cell lymphoma and skin tissues.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  64C1385

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13918于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 8792 Human

• Entrez Gene: 21934 Mouse

• Omim: 603499 Human

• SwissProt: Q9Y6Q6 Human

• SwissProt: O35305 Mouse

• Unigene: 204044 Human

• Unigene: 6251 Mouse

• 别名

• LOH18CR1 antibody

• Loss of heterozygosity 18 chromosomal region 1 antibody

• mRANK antibody

• ODFR antibody

• OFE antibody

• OPTB7 antibody

• Osteoclast differentiation factor receptor antibody

• OSTS antibody

• Paget disease of bone 2 antibody

• PDB 2 antibody

• PDB2 antibody

• RANK antibody

• Receptor activator of NF KB antibody

• Receptor activator of NF-KB antibody

• receptor activator of nuclear factor kappa B antibody

• TNF receptor superfamily member 11a antibody

• TNFRSF11A antibody

• TNR11_HUMAN antibody

• TRANCER antibody

• Tumor necrosis factor receptor superfamily member 11a NFKB activator antibody

• Tumor necrosis factor receptor superfamily member 11a activator of NFKB antibody

• Tumor necrosis factor receptor superfamily member 11A antibody

• CD 265 antibody

• CD265 antibody

• FEO antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)

  Formaldehyde-fixed, paraffin-embedded human skin tissue stained for RANK using ab13918 at 1/50 dilution in immunohistochemical analysis.

  See Abreview

• 

  Immunoprecipitation - Anti-RANK antibody [64C1385] (ab13918)

  Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)

  IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.