Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker

• 产品名称

  Anti-PSD95抗体[7E3-1B8] - Synaptic Marker
  参阅全部 PSD95 一抗

• 描述

  小鼠单克隆抗体[7E3-1B8] to PSD95 - Synaptic Marker

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ICC/IF, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  

• 免疫原

  Recombinant full length protein corresponding to Rat PSD95.

• 阳性对照

• ICC/IF: Rat primary hippocampal neurons; HaCaT cells; mouse backskin tissue section. WB: Rat brain membrane lysate; bovine brain tissue extract. Flow Cyt: K562 cells. IHC-P: Mouse backskin tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine)

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  7E3-1B8

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13552于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 463928 Human

• Unigene: 27256 Mouse

• Unigene: 9765 Rat

• Entrez Gene: 100137840 Cow

• Entrez Gene: 1742 Human

• Entrez Gene: 13385 Mouse

• Entrez Gene: 29495 Rat

• Omim: 602887 Human

• SwissProt: P78352 Human

• SwissProt: Q62108 Mouse

• SwissProt: P31016 Rat

• 别名

• Dlg4 antibody

• DLG4_HUMAN antibody

• FLJ97752 antibody

• FLJ98574 antibody

• Human post synaptic density protein 95 antibody

• Post synaptic density protein 95 antibody

• Postsynaptic density protein 95 antibody

• PSD 95 antibody

• PSD-95 antibody

• PSD95 antibody

• SAP 90 antibody

• SAP-90 antibody

• SAP90 antibody

• Synapse associated protein 90 antibody

• Synapse-associated protein 90 antibody

• Tax interaction protein 15 antibody

• Discs large homolog 4 antibody

• Disks large homolog 4 antibody

• DLG 4 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)This image is courtesy of an anonymous Abreview

  Central Panel: ab13552 staining PSD95 in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
  Left-hand panel: Homer GFP.
  Right-hand panel: Merge.

  See Abreview

• 

  Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

  Western blot analysis of bovine brain tissue extract, probed with ab13552. Western blot analysis of bovine brain tissue extract, probed with ab13552.

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  Flow Cytometry - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

  Overlay histogram showing K562 cells stained with ab13552 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13552, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

  ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.

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  Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

  Immunocytochemistry/ Immunofluorescence analysis of HaCaT cells labeling PSD95 with ab13552 at 1/100 dilution. Cells were fixed with 00% cold methanol for 10 minutes at -20°C. A FITC Goat Anti-Mouse antibody at 1/50 was used as secondary. The image shows Filamentouslike staining. 

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  Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

  Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552) at 1/1000 dilution + Rat brain membrane lysate.