Anti-CD40抗体
参阅全部 CD40 一抗
兔多克隆抗体to CD40
Rabbit
The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.
适用于: IHC-P, WBmore details
与反应: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Monkey
预测可用于: Chicken, Dog
Synthetic peptide:
CQPVTQEDGKESRISVQERQ
conjugated to KLH, corresponding to amino acids 258-277 of Human CD40.
Storage below -20°C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Peptide affinity purified.
多克隆
IgG
Abpromise™承诺保证使用ab13545于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (5) | Use at an assay dependent concentration. |
| WB | (2) | 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
Entrez Gene: 958 Human
Entrez Gene: 21939 Mouse
Omim: 109535 Human
SwissProt: P25942 Human
SwissProt: P27512 Mouse
Unigene: 472860 Human
Unigene: 271833 Mouse
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Western blot - Anti-CD40 antibody (ab13545)
All lanes : Anti-CD40 antibody (ab13545) at 1/200 dilution
Lane 1 : ESK4 cell lysate
Lane 2 : Molt 4 cell lysate
Predicted band size: 34 kDa
Western blot - Anti-CD40 antibody (ab13545)This image is courtesy of an anonymous Abreview
All lanes : Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1 : Human B chronic lymphocytic leukaemia cells, nuclear fraction
Lane 2 : Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction
Lane 3 : Human B chronic lymphocytic leukaemia cells, membrane fraction
Lane 4 : Human B chronic lymphocytic leukaemia cells, cytosolic fraction
Lysates/proteins at 18 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDawhy is the actual band size different from the predicted?
Exposure time: 3 minutes
The primary antibody was incubated with the membrane for 16 hours at 4°C.
Western blot - Anti-CD40 antibody (ab13545)
Anti-CD40 antibody (ab13545) at 1/1000 dilution + Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 8 minutes
Western blot - Anti-CD40 antibody (ab13545)
All lanes : Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lanes 1-2 : Control human HCAEC cells - whole cell lysate
Lanes 3-4 : Treated human HCAEC cells - whole cell lysate
Secondary
All lanes : HRP conjugated Goat Anti-Rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDawhy is the actual band size different from the predicted?
Exposure time: 20 seconds
This image is courtesy of an Abreview submitted on 10 August 2005.
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