Anti-Calnexin抗体
参阅全部 Calnexin 一抗
兔多克隆抗体to Calnexin
Rabbit
Recognizes ER membrane, mitochondria and cis-Golgi
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Rat, Human
Synthetic peptide:
TAPPSSPKVTYKAPVPTGE
conjugated to KLH, corresponding to amino acids 50-68 of Human Calnexin
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: Whole serum
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab13505于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/100. | |
| ICC/IF | 1/100. | |
| WB | 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
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Unigene: 248827 Mouse
Unigene: 1762 Rat
Entrez Gene: 821 Human
Entrez Gene: 12330 Mouse
Omim: 114217 Human
SwissProt: P27824 Human
SwissProt: P35564 Mouse
Unigene: 567968 Human
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IP90 antibody
Major histocompatibility complex class I antigen-binding protein p88 antibody
p90 antibody
Calnexin antibody
CALX_HUMAN antibody
CANX antibody
Western blot - Anti-Calnexin antibody (ab13505)
Anti-Calnexin antibody (ab13505) at 1/1000 dilution + Mixture of tissue lysates prepared from Rat tissues.
Predicted band size: 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab13505)
ab13505 staining Calnexin - ER membrane marker in mouse back skin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. The primary antibody was used at 1/100 dilution and a Fluorophore conjugated goat anti rabbit secondary at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab13505)
ab13505 staining Calnexin - ER membrane marker in mouse colitis colon tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated anti rabbit was used at 1/10 dilution as secondary.
Western blot - Anti-Calnexin antibody (ab13505)
Anti-Calnexin antibody (ab13505) at 1/2000 dilution ((in TBS with 0.05% TW-20)) + HeLa cell lysate
Secondary
An HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 90 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody (ab13505)
ab13505 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.
