Anti-Calnexin - ER膜marker抗体- ER Marker
参阅全部 Calnexin 一抗
兔多克隆抗体to Calnexin - ER膜marker - ER Marker
Rabbit
Weak reactivity with Chicken, Fruitfly (Drosophila melanogaster) and Xenopus laevis.
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Dog Calnexin aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P24643
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Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab13504于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| ICC/IF | (5) | |
| WB | (3) |
应用说明
Flow Cyt: 1/100.
ICC: 1/200.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 18621691).
IP: 1/100.
WB: 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 821 Human
Entrez Gene: 12330 Mouse
Omim: 114217 Human
SwissProt: P27824 Human
SwissProt: P35564 Mouse
Unigene: 567968 Human
Unigene: 248827 Mouse
Unigene: 1762 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Immunohistochemistry analysis using ab13504 at 1/100 for 1 hour at RT in mouse backskin. Fixed by Bouin’s Fixative Solution. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/50 for 1 hour at RT.
Western blot - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
All lanes : Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504) at 1/2000 dilution
All lanes : Mixture of rat tissue lysates
Predicted band size: 90 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/100 for 12 hours at 4°C in HaCaT cells. Fixed by Cold 100% methanol at -20C for 10 minutes. Secondary Antibody: FITC Goat Anti-Rabbit at 1/50 for 1-2 hours at RT in dark. /
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Heat Shocked at 42°C for 1h.
