Anti-Superoxide Dismutase 1抗体
参阅全部 Superoxide Dismutase 1 一抗
兔多克隆抗体to Superoxide Dismutase 1
Rabbit
适用于: IHC-P, ELISA, IHC-Fr, ICC/IF, IP, WBmore details
与反应: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, African green monkey, Coral, Mollusk
Synthetic peptide corresponding to Human Superoxide Dismutase 1.
Database link: P00441
WB: HeLa (Human epithelial cell line from cervix adenocarcinoma)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
This antibody was purified on an antigen coupled sepharose column.
多克隆
IgG
Abpromise™承诺保证使用ab13498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use at an assay dependent concentration. | |
ELISA | Use at an assay dependent concentration. See Downs et al 2002. | |
IHC-Fr | (3) | Use at an assay dependent concentration. |
ICC/IF | (6) | Use at an assay dependent concentration. See Abreview. |
IP | Use at an assay dependent concentration. See Shinder et al 2001. | |
WB | (13) | 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 16 kDa). |
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Omim: 147450 Human
SwissProt: P61851 Drosophila melanogaster
SwissProt: P00441 Human
SwissProt: P08228 Mouse
SwissProt: P09212 Rabbit
SwissProt: P09670 Sheep
Unigene: 926 Drosophila melanogaster
Unigene: 443914 Human
Unigene: 276325 Mouse
Unigene: 466779 Mouse
Unigene: 6059 Rat
Entrez Gene: 39251 Drosophila melanogaster
Entrez Gene: 6647 Human
Entrez Gene: 20655 Mouse
Entrez Gene: 100009313 Rabbit
Entrez Gene: 100270717 Sheep
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)
Anti-Superoxide Dismutase 1 antibody (ab13498) at 0.2 µg/ml (2 hrs at RT) + HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 15 µg
Secondary
Donkey Anti-Rabbit IgG HRP 1 hr at RT
Predicted band size: 16 kDa
Observed band size: 23 kDawhy is the actual band size different from the predicted?
Block: 1.5% BSA.
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