Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker

• 产品名称

  Anti-Syntaxin 6抗体[3D10] - Golgi膜Marker
  参阅全部 Syntaxin 6 一抗

• 描述

  小鼠单克隆抗体[3D10] to Syntaxin 6 - Golgi膜Marker

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, WB, IPmore details

• 种属反应性

  与反应: Mouse, Rat
  

• 免疫原

  Recombinant full length protein corresponding to Rat Syntaxin 6.
  Database link: Q63635

• 表位

  The epitope for this antibody has been localized to the amino terminus (residues 1-25) of Syntaxin 6.

• 阳性对照

• Mouse Brain Tissue Extract, rat Brain Tissue Extract.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3173630 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Purified from TCS

• 克隆

  单克隆

• 克隆编号

  3D10

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab12370于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 58244 Mouse

• Entrez Gene: 60562 Rat

• SwissProt: Q9JKK1 Mouse

• SwissProt: Q63635 Rat

• Unigene: 465911 Mouse

• Unigene: 66264 Mouse

• Unigene: 100217 Rat

• 别名

• Laminin-1 subunit gamma antibody

• Laminin-10 subunit gamma antibody

• Laminin-11 subunit gamma antibody

• STX 6 antibody

• STX6 antibody

• STX6_HUMAN antibody

• Syntaxin-6 antibody

• Syntaxin6 antibody

• LAMB2 antibody

• LAMC1 antibody

• Laminin B2 chain antibody

• 

  Western blot - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)

  All lanes : Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370) at 1 µg/ml
  
  Lane 1 : Brain (Mouse) Tissue Lysate
  Lane 2 : Brain (Rat) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 30.6 kDa
  Observed band size: 30.6 kDa
  Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 8 minutes
  
  
  

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)Image from Jonas MC et al, J Cell Sci. 2010 Oct 1;123(Pt 19):3378-88. Epub 2010 Sep 7, Fig 1, doi:10.1242/jcs.068841.

  Intracellular membranes from CHO cells were separated on a 10–24% discontinuous Nycodenz gradient. Western Blotting was then performed using a range of antibodies including ab12370 at a 1/1000 dilution. An HRP-conjugated anti-mouse secondary was used at a 1/6000 dilution.

  This image was generated using the ascites version of the product.

• 

  Immunoprecipitation - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)

  Syntaxin 6 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 10µg of Mouse monoclonal to Syntaxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12370.
  Secondary: Protein G-HRP at 1/500 dilution.
  Band: 25kDa:Syntaxin 6.

  This image was generated using the ascites version of the product.

• 

  Flow Cytometry - Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)

  Overlay histogram showing PC12 cells stained with ab12370 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12370, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

  This image was generated using the ascites version of the product.