Anti-Kaiso antibody [6F / 6F8]

• 产品名称

  Anti-Kaiso抗体[6F / 6F8]
  参阅全部 Kaiso 一抗

• 描述

  小鼠单克隆抗体[6F / 6F8] to Kaiso

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat, Chicken, Hamster, Dog

• 免疫原

  Fusion protein corresponding to Human Kaiso.

• 阳性对照

• CSML0 cells (IP then Western). Flow Cyt (Intra): HeLa cells.

• 常规说明

  We can conjugate this antibody to FITC for you (please see ab150256 for details).

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine
  
  Contains 0.4M Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  6F / 6F8

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab12723于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 10009 Human

• Entrez Gene: 56805 Mouse

• Entrez Gene: 501506 Rat

• Omim: 300329 Human

• SwissProt: Q86T24 Human

• SwissProt: Q8BN78 Mouse

• Unigene: 143604 Human

• Unigene: 391248 Mouse

• 别名

• KAISO_HUMAN antibody

• Transcriptional regulator Kaiso antibody

• ZBTB 33 antibody

• Zbtb33 antibody

• Zinc finger and BTB domain containing 33 antibody

• Zinc finger and BTB domain-containing protein 33 antibody

• Zinc finger transcription factor Kaiso antibody

• ZNF 348 antibody

• ZNF Kaiso antibody

• ZNF348 antibody

• Kaiso antibody

• Kaiso protein antibody

• Kaiso transcription factor antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

  Immunofluorescence using clone 6F / 6F8 on C2C12 cells.

• 

  Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

  Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.

  Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.

• 

  Flow Cytometry (Intracellular) - Anti-Kaiso antibody [6F / 6F8] (ab12723)

  Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] (ab12723)Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.

  Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.

• 

  Western blot - Anti-Kaiso antibody [6F / 6F8] (ab12723)Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.

  Representative western blot detecting Kaiso in ARPE-19 cells using ab12723.