Anti-PIWIL1抗体
参阅全部 PIWIL1 一抗
兔多克隆抗体to PIWIL1
Rabbit
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.2 - 0.7 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab12337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). ab12337 detects a 99 kDa band in both mouse and rat testis lysates (positive controls). Non-specific bands at 102 kDa were also observed in mouse and rat liver lysates (negative controls). Read More |
| ICC/IF | Use a concentration of 10 µg/ml. |
Entrez Gene: 9271 Human
Entrez Gene: 57749 Mouse
Omim: 605571 Human
SwissProt: Q96J94 Human
SwissProt: Q9JMB7 Mouse
Unigene: 405659 Human
Unigene: 272720 Mouse
PIWI antibody
Piwi homolog antibody
Piwi like 1 (drosophila) antibody
Piwi like 1 antibody
Piwi-like protein 1 antibody
PIWIL1 antibody
PIWL1_HUMAN antibody
HIWI antibody
MIWI antibody
Piwi (Drosophila) like 1 antibody
Western blot - Anti-PIWIL1 antibody (ab12337)
All lanes : Anti-PIWIL1 antibody (ab12337) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lane 4 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 102 kDa (possible non-specific binding)
ab12337 gave a positive signal in both mouse and rat testis lysates (positive controls). Bands were also observed in mouse and rat liver lysates (negative controls) that were higher in size to that observed in testis.
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody (ab12337)
ICC/IF image of ab12337 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12337 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
