Anti-JLP抗体
参阅全部 JLP 一抗
兔多克隆抗体to JLP
Rabbit
适用于: IHC-P, WB, IPmore details
与反应: Mouse, Human
预测可用于: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
Synthetic peptide within Human JLP aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60271
IP: HeLa nuclear extract. WB: HeLa, 293T,Jurkat, 3T3 cell lysates IHC-P: Human testis, and seminoma tissues
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
This antibody is affinity purified.
多克隆
IgG
Abpromise™承诺保证使用ab12331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | (1) | 1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa. |
| IP | Use a concentration of 2 - 5 µg/ml. |
Entrez Gene: 9043 Human
Entrez Gene: 70834 Mouse
Omim: 605430 Human
SwissProt: O60271 Human
SwissProt: Q58A65 Mouse
SwissProt: Q8CJC2 Mouse
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Western blot - Anti-JLP antibody (ab12331)
All lanes : Anti-JLP antibody (ab12331) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 156.8 kDa
Immunoprecipitation - Anti-JLP antibody (ab12331)
Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). ab12331 was used for immunoprecipitation at 3 µg/mg lysate and used for western blot at 1 µg/ml. Lanes 1 and 2 - ab12331 IP, Lane 3 - control IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12331)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human seminoma using ab12331 at 1/1000 with and HRP- conjugated goat anti-rabbit secondary antibody.
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