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Anti-RPA70 antibody

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  • 产品名称

    Anti-RPA70抗体
    参阅全部 RPA70 一抗

  • 描述

    兔多克隆抗体to RPA70

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-PWBmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan

  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human replication protein A1, 70kDa encoded within exon 12 (LocusLink ID 6117).

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

  • 浓度

    • 50 µg 浓度为 1 mg/ml

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified using the peptide immobilized on solid support.

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab12320于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
IHC-P

1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

WB

1/5000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

  • 数据库链接

  • 别名

    • HSSB antibody

    • Human single stranded DNA binding protein antibody

    • MST075 antibody

    • MSTP075 antibody

    • p70 antibody

    • REPA1 antibody

    • Replication factor A antibody

    • Replication factor A protein 1 antibody

    • Replication protein A 70 kDa DNA-binding subunit antibody

    • Replication protein A 70kDa DNA binding subunit antibody

    • Replication protein A1 70kDa antibody

    • Replication protein A1 antibody

    • RF A antibody

    • RF-A protein 1 antibody

    • RFA antibody

    • RFA1_HUMAN antibody

    • RP A antibody

    • RP-A p70 antibody

    • RPA 70 antibody

    • RPA antibody

    • rpa1 antibody

    • Single stranded binding protein 70 antibody

    • Single-stranded DNA-binding protein antibody

    • Dmrpa1 antibody

    • Drosophila Replication Protein A antibody

    • DRPA antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody (ab12320)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA70 antibody (ab12320)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RPA70 with ab12320 at 1/1000 (1 µg/ml). Detection: DAB.

  • Western blot - Anti-RPA70 antibody (ab12320)

    Western blot - Anti-RPA70 antibody (ab12320)

    Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells. Antibody: ab12320 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.

     

    Detection of Human RPA70 by Western Blot.
    Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells.
    Antibody: ab12320 used at the indicated concentrations.
    Detection: Chemiluminescence with a 10 second exposure.