Anti-PLK1 antibody [AZ34]

• 产品名称

  Anti-PLK1抗体[AZ34]
  参阅全部 PLK1 一抗

• 描述

  小鼠单克隆抗体[AZ34] to PLK1

• 宿主

  Mouse

• 特异性

  The cross-reactivity of this antibody regards the various isoforms of PLK is not known.

• 经测试应用

  适用于: WBmore details
  不适用于: ICC/IF

• 种属反应性

  与反应: Human, Xenopus laevis
  

• 免疫原

  Recombinant full length protein (Xenopus laevis).

• 阳性对照

• Hela cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 50 µg 浓度为 1 mg/ml

• 50 µg 浓度为 0.5 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  AZ34

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab12211于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for ICC/IF.

• 数据库链接

• Entrez Gene: 5347 Human

• Entrez Gene: 380481 Xenopus laevis

• Omim: 602098 Human

• SwissProt: P53350 Human

• SwissProt: P70032 Xenopus laevis

• Unigene: 592049 Human

• Unigene: 28141 Xenopus laevis

• 别名

• PLK-1 antibody

• plk1 antibody

• PLK1_HUMAN antibody

• Polo like kinase 1 antibody

• Polo-like kinase 1 antibody

• Serine/threonine protein kinase 13 antibody

• Serine/threonine protein kinase PLK1 antibody

• Serine/threonine-protein kinase 13 antibody

• Serine/threonine-protein kinase PLK1 antibody

• STPK 13 antibody

• STPK13 antibody

• Cell cycle regulated protein kinase antibody

• PLK 1 antibody

• PLK antibody

• 

  Western blot - Anti-PLK1 antibody [AZ34] (ab12211)This image is courtesy of Julian Gannon, CRUK

  Western blot using ab12211.

  Lane 1: Mitotic CSF Xenopus egg extract
  Lane 2: Interphase Xenopus egg extract
  Lane 3: HeLa cell extract

  Note that the phosphorylated form of Plx from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.

  Western blot using ab12211.

  Lane 1: Mitotic CSF Xenopus egg extract
  Lane 2: Interphase Xenopus egg extract
  Lane 3: HeLa cell extract
  Note that the phosphorylated form of Plx from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.

• 

  Western blot - Anti-PLK1 antibody [AZ34] (ab12211)

  Anti-PLK1 antibody [AZ34] (ab12211) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size: 68 kDa
  Observed band size: 57 kDawhy is the actual band size different from the predicted?