Anti-FOXO3A抗体
参阅全部 FOXO3A 一抗
兔多克隆抗体to FOXO3A
Rabbit
适用于: IHC-P, ICC/IFmore details
与反应: Human
预测可用于: Rat
Synthetic peptide: C-GLNVGNFTGAKQASSQ, with N-terminally added cysteine, conjugated to maleimide activated KLH, corresponding to amino acids 653-668 of Human FOXO3A.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
浓度
50 µl 浓度为 1.1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab12162于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use a concentration of 10 µg/ml. |
| ICC/IF | (2) | 1/200. |
Entrez Gene: 2309 Human
Omim: 602681 Human
SwissProt: O43524 Human
Unigene: 220950 Human
FKHR2 antibody
FKHRL 1 antibody
FKHRL1 antibody
FKHRL1P2 antibody
Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
Forkhead box O3 antibody
Forkhead box O3A antibody
Forkhead box protein O3 antibody
Forkhead box protein O3A antibody
Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
Forkhead homolog (rhabdomyosarcoma) like 1 antibody
Forkhead in rhabdomyosarcoma like 1 antibody
Forkhead in rhabdomyosarcoma-like 1 antibody
FOX O3A antibody
FOXO2 antibody
foxo3 antibody
FOXO3_HUMAN antibody
FOXO3A antibody
MGC12739 antibody
MGC31925 antibody
AF6q21 antibody
AF6q21 protein antibody
DKFZp781A0677 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab12162)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue labelling FOXO3a with ab12162 at 10 µg/mL.
Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A antibody (ab12162)
Immunocytochemistry/immunofluorescence analysis of human cervix carcinoma cells labelling FOXO3A with ab[12162 at 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A antibody (ab12162)This image is courtesy of an anonymous Abreview
ab12162 staining FOXO3A in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.03% Triton X-100 and blocked with 5% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 1 hour at 25°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab12162)This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, staining FOXO3A with ab12162.
Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. A biotinylated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
