Anti-gamma Catenin antibody [15F11]

• 产品名称

  Anti-gamma Catenin抗体[15F11]
  参阅全部 gamma Catenin 一抗

• 描述

  小鼠单克隆抗体[15F11] to gamma Catenin

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat

• 免疫原

  Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-P: FFPE normal human skin tissue sections. ICC/IF: A431 cells. Flow Cyt (Intra): HeLa cells. WB: HeLa, A431 cells

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  15F11

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab12083于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 299774 Mouse

• Unigene: 11255 Rat

• Entrez Gene: 3728 Human

• Entrez Gene: 16480 Mouse

• Entrez Gene: 81679 Rat

• Omim: 173325 Human

• SwissProt: P14923 Human

• SwissProt: Q02257 Mouse

• SwissProt: Q6P0K8 Rat

• Unigene: 514174 Human

• 别名

• catenin (cadherin-associated protein) gamma (80kD) antibody

• Catenin gamma antibody

• CTNNG antibody

• Desmoplakin 3 antibody

• Desmoplakin III antibody

• Desmoplakin-3 antibody

• Desmoplakin3 antibody

• DesmoplakinIII antibody

• DP 3 antibody

• DP III antibody

• DP3 antibody

• DPIII antibody

• gamma catenin antibody

• Junction plakoglobin antibody

• JUP antibody

• OTTHUMP00000164732 antibody

• OTTHUMP00000164735 antibody

• OTTHUMP00000164738 antibody

• PDGB antibody

• PKGB antibody

• PLAK_HUMAN antibody

• PLAKOGLOBIN antibody

• ARVD 12 antibody

• ARVD12 antibody

• Catenin (cadherin associated protein), gamma 80kDa antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15F11] (ab12083)

  ab12083 staining gamma Catenin in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab12083 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [15F11] (ab12083)

  IHC image of gamma Catenin staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab12083, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Anti-gamma Catenin antibody [15F11] (ab12083)

  All lanes :
  
  Lane 1 : A431 whole cell lysate
  Lane 2 : HeLa whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 81.5 kDa
  
  
  
  

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before ab12083 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-gamma Catenin antibody [15F11] (ab12083)

  Anti-gamma Catenin antibody [15F11] (ab12083) at 1 µg/ml + Mouse Liver Tissue Lysate at 20 µg
  
  Performed under reducing conditions.
  
  Predicted band size: 81.5 kDa
  Additional bands at: 25 kDa (possible IgG), 55 kDa (possible IgG)
  
  
  
  

  This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab12083 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/ml and a 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [15F11] (ab12083)

  Overlay histogram showing HeLa cells stained with ab12083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.