Anti-Mre11抗体- ChIP Grade
参阅全部 Mre11 一抗
兔多克隆抗体to Mre11 - ChIP Grade
Rabbit
A BLAST analysis was used to suggest that little cross reactivity should be expected with this protein from other sources.
适用于: ChIPmore details
与反应: Saccharomyces cerevisiae
Synthetic peptide:
C-SQAESSKPTSKPK
conjugated to KLH, corresponding to amino acids 578-592 of S. cerevisiae Mre11.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.17 mg/ml
Immunogen affinity purified
This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab12159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP | Use at an assay dependent dilution. |
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ChIP - Anti-Mre11 antibody - ChIP Grade (ab12159)
A Saccharomyces cerevisiae strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorometer.
