Anti-ARPC2抗体
参阅全部 ARPC2 一抗
山羊多克隆抗体to ARPC2
Goat
This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601.
适用于: IHC-P, WB, ICC/IFmore details
与反应: Human
预测可用于: Rat, Zebrafish
Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.
WB: Caco-2, HeLa and Jurkat cell lysates. IHC-P: Human Muscle lysates. ICC/IF: HeLa and A431 cells
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab11798于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa. A 1 hour primary incubation at room temperature is recommended for this product. | |
| ICC/IF | Use a concentration of 10 µg/ml. PubMed ID: 22021442 |
Entrez Gene: 10109 Human
Entrez Gene: 327068 Zebrafish
Omim: 604224 Human
SwissProt: O15144 Human
Unigene: 529303 Human
Unigene: 93317 Rat
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Western blot - Anti-ARPC2 antibody (ab11798)
Lane 1 : Anti-ARPC2 antibody (ab11798) at 0.5 µg/ml
Lanes 2-3 : Anti-ARPC2 antibody (ab11798) at 0.1 µg/ml
Lane 1 : CACO-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Observed band size: 34-35 kDawhy is the actual band size different from the predicted?
Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)
ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6).
Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)
Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
