Anti-NEAS antibody [D8B7]

• 产品名称

  Anti-NEAS抗体[D8B7]
  参阅全部 NEAS 一抗

• 描述

  小鼠单克隆抗体[D8B7] to NEAS

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, ICC, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human, Drosophila melanogaster
  

• 免疫原

  Full length native protein (purified) corresponding to Human NEAS.

• 阳性对照

• IHC-P: Mouse and Rat brain tissue. ICC: 3T3, HeLa cells.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 21/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.20
  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  D8B7

• 同种型

  IgG2b

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11755于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 6709 Human

• Entrez Gene: 64159 Rat

• Omim: 182810 Human

• SwissProt: Q13813 Human

• SwissProt: P16546 Mouse

• SwissProt: P16086 Rat

• Unigene: 372331 Human

• Unigene: 204969 Mouse

• Unigene: 5812 Rat

别名

• (ALPHA)II-SPECTRIN antibody

• Alpha II Spectrin antibody

• Alpha-II spectrin antibody

• brain antibody

• EIEE5 antibody

• FLJ17738 antibody

• FLJ44613 antibody

• Fodrin alpha chain antibody

• Fodrin, alpha antibody

• NEAS antibody

• Non erythrocytic spectrin alpha antibody

• non-erythroid alpha chain antibody

• SPECA antibody

• Spectrin alpha chain antibody

• Spectrin alpha chain brain antibody

• Spectrin alpha non erythrocytic 1 antibody

• Spectrin antibody

• Spectrin non erythroid alpha chain antibody

• Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody

• Spectrin, nonerythroid, alpha subunit antibody

• Spna2 antibody

• SPTA 2 antibody

• SPTA2 antibody

• SPTA2_HUMAN antibody

• SPTAN 1 antibody

• SPTAN1 antibody

• 

  Western blot - Anti-NEAS antibody [D8B7] (ab11755)

  Lanes 2-6 : Anti-NEAS antibody [D8B7] (ab11755) at 0.5 µg/ml
  
  Lane 1 : MW marker
  Lane 2 : Human Brain lysate at 20 µg
  Lane 3 : Mouse Brain lysate at 20 µg
  Lane 4 : Rat Brain lysate at 20 µg
  Lane 5 : 3T3 cell lysate at 20 µg
  Lane 6 : HeLa cell lysate at 20 µg
  
  Secondary
  Lanes 2-6 : HRP labeled goat anti-mouse IgG
  
  Predicted band size: 297 kDa
  
  

• 

  Immunocytochemistry - Anti-NEAS antibody [D8B7] (ab11755)

  ICC staining of purified ab11755 on 3T3 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [D8B7] (ab11755)

  IHC staining of purified ab11755 on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm

• 

  Western blot - Anti-NEAS antibody [D8B7] (ab11755)

  Lanes 2-3 : Anti-NEAS antibody [D8B7] (ab11755) at 0.1 µg/ml
  
  Lane 1 : MW marker
  Lane 2 : Drosophila head lysate at 20 µg
  Lane 3 : Drosophila S2 (embryonic) cell lysate at 20 µg
  
  Secondary
  Lanes 2-3 : HRP-labeled goat anti-mouse IgG
  
  Predicted band size: 297 kDa
  
  

• 

  Immunocytochemistry - Anti-NEAS antibody [D8B7] (ab11755)

  ICC staining of purified ab11755 on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [D8B7] (ab11755)

  IHC staining of purified ab11755 on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm

• 

  Western blot - Anti-NEAS antibody [D8B7] (ab11755)This image is courtesy of an anonymous Abreview

  Anti-NEAS antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg
  
  Secondary
  HRP conjugated goat anti-mouse at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 297 kDa
  Observed band size: 250 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 3 minutes
  
  
  

  This image was generated using the ascites version of the product.

  See Abreview

• 

  Immunocytochemistry - Anti-NEAS antibody [D8B7] (ab11755)

  IF using ab11755.

  This image was generated using the ascites version of the product. 

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [D8B7] (ab11755)This image is courtesy of an anonymous Abreview

  ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

  This image was generated using the ascites version of the product. 

  See Abreview