Anti-ERp29抗体
参阅全部 ERp29 一抗
兔多克隆抗体to ERp29
Rabbit
适用于: IP, Flow Cyt, IHC-Fr, WB, ICC/IFmore details
与反应: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Non human primates
Recombinant full length protein (His-tag) corresponding to Rat ERp29.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab11420于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 17451556 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. | |
| IHC-Fr | (1) | Use at an assay dependent concentration. PubMed: 17652758 |
| WB | (3) | 1/2500. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate. |
| ICC/IF | 1/500. Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420. |
Entrez Gene: 10961 Human
Entrez Gene: 67397 Mouse
Omim: 602287 Human
SwissProt: P30040 Human
SwissProt: P57759 Mouse
Unigene: 75841 Human
Unigene: 154570 Mouse
Unigene: 32904 Rat
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Western blot - Anti-ERp29 antibody (ab11420)
All lanes : Anti-ERp29 antibody (ab11420) at 1 µg/ml
Lane 1 : MDCK (canine kidney cell line) membrane-enriched extract
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) membrane-enriched extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) membrane-enriched extract
Lane 4 : A549 (human lung carcinoma cell line) membrane-enriched extract
Lane 5 : MCF7 (human breast adenocarcinoma cell line) membrane-enriched extract
Lane 6 : A431 (human epidermoid carcinoma cell line) membrane-enriched extract
Lane 7 : HEK-293 (human epithelial cell line from embryonic kidney) membrane-enriched extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
12% SDS-PAGE
Western blot - Anti-ERp29 antibody (ab11420)
Western blot of ERp29 in HEK cell lysate using ab11420.
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)
ICC/IF image of ab11420 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11420, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)
Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labeling ERp29 with ab11420.
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)
Immunocytochemistry/Immunofluorescence analysis of NS-1 cells labeling ERp29 with ab11420.
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)
Immunocytochemistry/Immunofluorescence analysis of p19 cells labeling ERp29 with ab11420.
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)
Immunocytochemistry/Immunofluorescence analysis of rat thyrocytes labeling ERp29 with ab11420.
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