Anti-gamma H2A.X (phospho S139)抗体
参阅全部 gamma H2A.X 一抗
兔多克隆抗体to gamma H2A.X (phospho S139)
Rabbit
Using IF, this antibody was shown to bind to a non-nuclear location in Hela cells.
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Human
预测可用于: Rabbit, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Bat
Synthetic peptide corresponding to Human gamma H2A.X (phospho S139).
Database link: 3014
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab11174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (5) | 1/1000 - 1/5000. |
| ICC/IF | (7) | 1/500 - 1/5000. |
| WB | (11) | 1/2000 - 1/10000. Detects a band of approximately 15 kDa. |
Entrez Gene: 3014 Human
Entrez Gene: 15270 Mouse
Omim: 601772 Human
SwissProt: P16104 Human
SwissProt: P27661 Mouse
Unigene: 477879 Human
Unigene: 245931 Mouse
H2A.X antibody
H2a/x antibody
H2AFX antibody
H2AX antibody
H2AX_HUMAN antibody
Histone H2A.X antibody
H2A histone family member X antibody
H2A histone family member X antibody
H2A.FX antibody
Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (ab11174)
ab11174 staining gamma H2A.X (phospho S139) in HeLa UV cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11174 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (ab11174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labeling gamma H2A.X (phospho S139) with ab11174 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.
Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174)
All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cells treated with 100 µM etoposide, whole cell lysate
Lane 2 : NIH/3T3 cells mock treated, whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP)
Exposure time: 3 seconds
Lower Panel: Rabbit anti-GAPDH antibody.
Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (ab11174)
Immunocytochemistry/Immunofluorescence analysis of neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) labelling H2A.X (phospho S139 with ab11174 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.
Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174)
All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cells treated with 100 µM etoposide, whole cell lysate
Lane 2 : Jurkat cells mock treated, whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP)
Exposure time: 10 seconds
Lower Panel shows western blot for total H2AX using rabbit anti-H2AX recombinant monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (ab11174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse CT26 colon carcinoma tissue labeling gamma H2A.X (phospho S139) with ab11174 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.
Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174)This image is courtesy of an anonymous Abreview
All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 1/5000 dilution
Lane 1 : HeLa nuclear lysate - untreated
Lane 2 : HeLa nuclear lysate - IR treated
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 17 kDawhy is the actual band size different from the predicted?
Exposure time: 30 seconds
Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (ab11174)This image is courtesy of an anonymous Abreview
ab11174 at 1/1000 staining human HeLa cells by ICC/IF. These cells express a gene that causes a DNA damage response, leading to H2AX phosphorylation. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary.
