Anti-CDKN2A/p14ARF antibody [ARF 4C6/4]

• 产品名称

  Anti-CDKN2A/p14ARF抗体[ARF 4C6/4]
  参阅全部 CDKN2A/p14ARF 一抗

• 描述

  小鼠单克隆抗体[ARF 4C6/4] to CDKN2A/p14ARF

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Bacteria CDKN2A/p14ARF.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  ARF 4C6/4

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab11048于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1029 Human

• Omim: 600160 Human

• SwissProt: P42771 Human

• SwissProt: Q8N726 Human

• 别名

• CDKN2 antibody

• CDKN2A antibody

• Cell cycle negative regulator beta antibody

• CMM2 antibody

• Cyclin dependent kinase 4 inhibitor A antibody

• Cyclin dependent kinase inhibitor 2A antibody

• INK4 antibody

• INK4a antibody

• Melanoma p16 inhibits CDK4 antibody

• MLM antibody

• MTS 1 antibody

• MTS1 antibody

• Multiple tumor suppressor 1 antibody

• p14 antibody

• p16 antibody

• p16 INK4a antibody

• p16INK4a antibody

• p19 antibody

• P19ARF antibody

• TP16 antibody

• ARF antibody

• CDK4 inhibitor p16 INK4 antibody

• CDK4I antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [ARF 4C6/4] (ab11048)

  ICC/IF image of ab11048 stained human MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11048, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2cells.

• 

  Flow Cytometry - Anti-CDKN2A/p14ARF antibody [ARF 4C6/4] (ab11048)

  Overlay histogram showing HeLa cells stained with ab11048 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11048, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.