Anti-UBE3A抗体
参阅全部 UBE3A 一抗
兔多克隆抗体to UBE3A
Rabbit
适用于: WB, IPmore details
与反应: Mouse, Human
预测可用于: Rabbit, Chicken, Guinea pig, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
Synthetic peptide corresponding to Human UBE3A aa 400-500.
Database link: Q05086
WB: HeLa, HEK293T, mouse NIH/3T3 cells, IP: HeLa.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10488于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). |
| IP | Use at 1-4 µg/mg of lysate. |
Entrez Gene: 7337 Human
Entrez Gene: 22215 Mouse
Omim: 601623 Human
SwissProt: Q05086 Human
SwissProt: O08759 Mouse
Unigene: 598862 Human
Unigene: 9002 Mouse
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Western blot - Anti-UBE3A antibody (ab10488)
All lanes : Anti-UBE3A antibody (ab10488) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 105 kDa
Exposure time: 3 minutes
Immunoprecipitation - Anti-UBE3A antibody (ab10488)
Immunoprecipitation of HeLa UBE3A with ab10488
Lane 1: ab10488
Lane 2: rabbit anti-UBE6A antibody which recognizes an upstream epitope
Lane 3: Control IgG
