Anti-Cdc27 antibody [AF3.1]

• 产品名称

  Anti-Cdc27抗体[AF3.1]
  参阅全部 Cdc27 一抗

• 描述

  小鼠单克隆抗体[AF3.1] to Cdc27

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27.

• 阳性对照

• HeLa cell nuclear extract.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 100 µg 浓度为 0.5 mg/ml

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  AF3.1

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab10538于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 996 Human

• Entrez Gene: 217232 Mouse

• Omim: 116946 Human

• SwissProt: P30260 Human

• SwissProt: A2A6Q5 Mouse

• Unigene: 463295 Human

• Unigene: 89845 Mouse

• 别名

• Anaphase Promoting Complex Subunit 3 antibody

• Anaphase-promoting complex subunit 3 antibody

• APC 3 antibody

• APC3 antibody

• Cdc 27 antibody

• Cdc27 antibody

• CDC27 homolog antibody

• CDC27_HUMAN antibody

• CDC27Hs antibody

• Cell Division Cycle 27 antibody

• Cell division cycle protein 27 homolog antibody

• D0S1430E antibody

• D17S978E antibody

• H NUC antibody

• H-NUC antibody

• HNUC antibody

• Nuc2 homolog antibody

• ANAPC3 antibody

• Anaphase Promoting Complex 3 antibody

• Anaphase promoting complex protein 3 antibody

• 

  Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)

  All lanes : Anti-Cdc27 antibody [AF3.1] (ab10538)
  
  Lane 1 : HeLa cell lysate
  Lane 2 : 293 cell lysate
  Lane 3 : Mouse myeloid cell lysate
  
  Predicted band size: 92 kDa
  Observed band size: 80 kDawhy is the actual band size different from the predicted?
  
  
  
  We have no more information on how this image was prepared by the academic who produced the antibody.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)

  IHC image of ab10538 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10538, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)

  Overlay histogram showing K562 cells stained with ab10538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.