Anti-RSK1 p90 (phospho S221)抗体
参阅全部 RSK1 p90 一抗
兔多克隆抗体to RSK1 p90 (phospho S221)
Rabbit
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Rat
Synthetic peptide corresponding to Human RSK1 p90 (phospho S221).
WB: A431 cells. IHC-P: Human brain tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
PBS is Ca2+ and Mg2+ free
Immunogen affinity purified
Purified from rabbit serum by epitope-specific affinity chromatography.
多克隆
IgG
Abpromise™承诺保证使用ab10695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. | |
| WB | 1/1000. Predicted molecular weight: 90 kDa. |
Entrez Gene: 6195 Human
Omim: 601684 Human
SwissProt: Q15418 Human
Unigene: 149957 Human
Unigene: 34915 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK1 p90 (phospho S221) antibody (ab10695)
Paraffin embedded human brain tissue (right) stained for RSK1 using ab10695 at 1/20 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
Western blot - Anti-RSK1 p90 (phospho S221) antibody (ab10695)
Peptide Competition - Lysates prepared from A431 cells left unstimulated (1) or stimulated with 100 ng/mL PMA for 30 minutes at 37oC (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% non-fat dried milk-TBST buffer for one hour at room temperature and incubated with ab10695 for one hour at room temperature in a 1% non-fat dried milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate in 1% non-fat dried milk-TBST buffer, and bands were detected by chemiluminescence. The data show induction of ab10695 signal by PMA treatment and that the peptide corresponding to this site blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Co
