Anti-SMC2抗体
参阅全部 SMC2 一抗
兔多克隆抗体to SMC2
Rabbit
适用于: WB, IHC-Pmore details
与反应: Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human SMC2 aa 50-150. The epitope maps to a region between residues 50 and 100 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Database link: O95347
WB: Mock transfected or SMC2 transfected HEK-293T whole cell lysates. IHC-P: Human cerebral cortex tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10399于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa). |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 10592 Human
Entrez Gene: 397800 Xenopus laevis
Omim: 605576 Human
SwissProt: O95347 Human
SwissProt: P50533 Xenopus laevis
Unigene: 119023 Human
Unigene: 928 Xenopus laevis
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Western blot - Anti-SMC2 antibody (ab10399)
All lanes : Anti-SMC2 antibody (ab10399) at 0.1 µg/ml
Lane 1 : E: Mock transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : T: SMC2 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 90 µg per lane.
Predicted band size: 143 kDa
Exposure time: 10 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (ab10399)
IHC image of ab10399 staining in normal human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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