Anti-Apc1 (phospho S355)抗体
参阅全部 Apc1 一抗
兔多克隆抗体to Apc1 (phospho S355)
Rabbit
This antibody is specific for phosphorylated human APC1 protein at the pS355 residue. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from chimpanzee and chicken is expected as the sequence of the immunogen only varies by one amino acid in from these sources (89% homology). Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog
Synthetic peptide corresponding to Human Apc1 aa 350-450 (phospho S355) conjugated to keyhole limpet haemocyanin.
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC1 protein at the pS355 residue.
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
多克隆
IgG
Abpromise™承诺保证使用ab10923于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). |
Entrez Gene: 64682 Human
Entrez Gene: 17222 Mouse
Omim: 608473 Human
SwissProt: Q9H1A4 Human
SwissProt: P53995 Mouse
Unigene: 436527 Human
Unigene: 277408 Mouse
Unigene: 449185 Mouse
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Western blot - Anti-Apc1 (phospho S355) antibody (ab10923)
All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution
Lane 1 : HeLa S3 whole cell lysate (asynchronous cells)
Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine)
Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG [H&L] at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDa
Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division.
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