Anti-Pumilio 2抗体
参阅全部 Pumilio 2 一抗
兔多克隆抗体to Pumilio 2
Rabbit
适用于: WB, IPmore details
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human Pumilio 2 aa 100-200.
Database link: 23369
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab10361于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/10000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 127 kDa). | |
IP | Use at 5-10 µg/mg of lysate. |
Entrez Gene: 23369 Human
Entrez Gene: 80913 Mouse
Omim: 607205 Human
SwissProt: Q8TB72 Human
SwissProt: Q80U58 Mouse
Unigene: 467824 Human
Unigene: 341243 Mouse
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Western blot - Anti-Pumilio 2 antibody (ab10361)
All lanes : Anti-Pumilio 2 antibody (ab10361) at 1/5000 dilution
Lane 1 : HeLa Whole cell lysate at 5 µg
Lane 2 : HeLa Whole cell lysate at 15 µg
Lane 3 : HeLa Whole cell lysate at 50 µg
Lane 4 : HEK293T Whole cell lysate at 50 µg
Lane 5 : NIH 3T3 Whole cell lysate at 50 µg
Predicted band size: 127 kDa
Detection: Chemiluminescence with exposure time of 10 seconds
Immunoprecipitation - Anti-Pumilio 2 antibody (ab10361)
Pumilio 2 was immunoprecipitated from HeLa whole cell lysate with 3 µg/mg lysate ab10361. Western blot was performed from the immunoprecipitate using ab10361 at 1 µg/ml dilution.
Lane 1: ab10361 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.