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Anti-Cardiac Troponin T antibody [1F11]

产品编号 : ab10214

英文名称 :

中文名称 : 抗体

产品等级 :

品牌 : Abcam

包装规格	交货周期	质量标准	目录价	会员专享价	数量
200ug			￥5924	登录后可见	- +

产品详情

产品名称
Anti-Cardiac Troponin T抗体[1F11]
参阅全部 Cardiac Troponin T 一抗
描述
小鼠单克隆抗体[1F11] to Cardiac Troponin T
宿主
Mouse
特异性
Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813)
经测试应用
适用于: Sandwich ELISA, ICCmore details
种属反应性
与反应: Human, Recombinant fragment
免疫原
Tissue, cells or virus corresponding to Human Cardiac Troponin T. Free human cardiac troponin T
表位
Peptide 146-160.
阳性对照

常规说明
This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
形式
Liquid
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
浓度

纯度
Protein A purified
纯化说明
Purity tested by electrophoresis.
Primary antibody说明
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
克隆
单克隆
克隆编号
1F11
骨髓瘤
Sp2/0
同种型
IgG2b

Abpromise™承诺保证使用ab10214于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Sandwich ELISA		Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
ICC	(2)	Use a concentration of 1 - 5 µg/ml.

Immunocytochemistry - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
Immunofluorescence staining of Cardiac Troponin T using ab10214 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab10214 at 1 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab10214 also gave a positive staining using 4% formaldehyde fixation (10 min).
Sandwich ELISA - Anti-Cardiac Troponin T antibody [1F11] (ab10214)
Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5µg/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5µg/ml.