Anti-Macrophage Inflammatory蛋白3 alpha抗体
参阅全部 Macrophage Inflammatory Protein 3 alpha 一抗
兔多克隆抗体to Macrophage Inflammatory蛋白3 alpha
Rabbit
适用于: IHC-P, ICC, ICC/IF, IHC-FoFr, WB, ELISA, Neutralising, Sandwich ELISAmore details
与反应: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Macrophage Inflammatory Protein 3 alpha. Highly pure (>98%) recombinant hMIP-3-alpha (human macrophage inflammatory protein-3 alpha)
Database link: P78556
IHC-P: Hepatocellular carcinoma and colon cancer tissues.
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab224188.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C
Shipped at 4°C. Store at -20ºC.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab9829于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) | Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| ICC | Use at an assay dependent concentration. PubMed: 24633013 | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20952674 | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 19305396 | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 10 kDa. To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More | |
| ELISA | Use at an assay dependent concentration. To detect hMIP-3-alpha by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha. Read More | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 ug/ml of this antibody is required. | |
| Sandwich ELISA | Use at an assay dependent concentration. To detect Human MIP-3α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271284 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3α. Read More |
Entrez Gene: 6364 Human
Entrez Gene: 20297 Mouse
Omim: 601960 Human
SwissProt: P78556 Human
SwissProt: O89093 Mouse
Unigene: 75498 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Macrophage Inflammatory Protein 3 alpha with ab9829 at 4 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue labelling Macrophage Inflammatory Protein 3 alpha with ab9829 at 4 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
ab9829 detecting Macrophage Inflammatory Protein 3 alpha using sandwich ELISA
