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Mouse IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-10 in Mouse in Biofluids, Cell Culture Media samples.
隐藏别名
Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF
Colorimetric
Plasma, Cell culture supernatant, Serum
Mouse
Sandwich (quantitative)
< 45 pg/mL
78.13 - 5000 pg/mL
Microplate
Abcam's Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Blue Ice
-20°C
-20°C
-20°C
Representative standard curve using ab100697
Representative standard curve using ab100697
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