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beta-Actin (8H10D10) Mouse mAb

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包装规格 交货周期 质量标准 目录价 会员专享价 数量
100 µl 现货2-3天 原装正品
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基础信息
存储条件 -20℃
产品详情

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Fluorescent Western1:1000
Simple Western™1:10 - 1:50
Immunohistochemistry (Paraffin)1:3000 - 1:12000
Immunofluorescence (Immunocytochemistry)1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized)1:800 - 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

beta-Actin (8H10D10) Mouse Monoclonal Antibody detects endogenous levels of total β-actin protein. Due to the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, this antibody may cross-react with cytoplasmic γ-actin. This antibody does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, Dog

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human β-actin.


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