| 存储条件 | -20℃ |
|---|
Human CXCL9 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CXCL9 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CMK, MIG, SCYB9, CXCL9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Small-inducible cytokine B9, HuMIG
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 20 pg/mL
8.23 - 6000 pg/mL
Microplate
Abcam's CXCL9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL9 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100595.
Representative Standard Curve using ab100595.
抱歉，暂无相关文献
