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Human MICA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MICA in Human in Biofluids, Cell Culture Media samples.
隐藏别名
PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 20 pg/mL
13.72 - 10000 pg/mL
Microplate
Abcam's MICA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MICA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MICA coated on a 96-well plate. Standards and samples are pipetted into the wells and MICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Standard curve with background signal subtracted (duplicates; +/- SD).
Representative Standard Curve using ab100592.
MICA measured in undiluted cell culture supernatants, U937 and THP-1 signals were below level of detection (13.7 pg x mL-1) (duplicates +/- SD).
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