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Human Fas Ligand ELISA Kit (APTL) is a Sandwich (quantitative) ELISA for the measurement of Human Fas Ligand (APTL) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 2 pg/mL
1.37 - 1000 pg/mL
Microplate
Abcam's Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
Representative Standard Curve using ab100515.
Representative Standard Curve using ab100515.
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