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Human CCL23 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CCL23 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
MIP3, MPIF1, SCYA23, CCL23, C-C motif chemokine 23, CK-beta-8, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, Small-inducible cytokine A23, CKB-8, MIP-3, MPIF-1
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 7 pg/mL
8.23 - 6000 pg/mL
Microplate
Abcam's CCL23 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL23 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CCL23 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CCL23 ELISA Kit (ab216169) from our SimpleStep ELISA® range.
Optimisation may be required with urine samples.
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Representative Standard Curve using ab100611.

Representative Standard Curve using ab100611.
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